Detection of cytomegalovirus DNA, viral antigens, and antibodies to it in patients after organ transplantation

Cytomegalovirus (CMV) DNA was determined by the PCR method, anti-CMV IgM and IgG were analyzed by enzyme immunoassay, and infective activity of CMV was evaluated by the rapid culture technique with monoclonal antibodies in the blood of patients, who underwent organ transplantation. In cases when the...

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Published inBulletin of experimental biology and medicine Vol. 127; no. 5; pp. 507 - 510
Main Authors Dolgikh, M. S., Vedyakov, A. M., Feoktistova, E. Yu, Adueva, S. M., Makarova, N. E., Kushch, A. A., Pustovoit, B.
Format Journal Article
LanguageEnglish
Published New York Springer Nature B.V 01.05.1999
Subjects
Blood
Cytomegalovirus
Deoxyribonucleic acid
DNA
Enzyme immunoassay
Immunoassay
Immunoglobulin G
Immunoglobulin M
Infection
Monoclonal antibodies
Polymerase chain reaction
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Summary:Cytomegalovirus (CMV) DNA was determined by the PCR method, anti-CMV IgM and IgG were analyzed by enzyme immunoassay, and infective activity of CMV was evaluated by the rapid culture technique with monoclonal antibodies in the blood of patients, who underwent organ transplantation. In cases when the results coincided, PCR was much more sensitive, while the rapid culture technique was more reliable in predicting the course and outcome of CMV disease. Enzyme immunoassay failed to detect active infection by the moment of examination.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0007-4888
1573-8221
DOI:10.1007/BF02434954