Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis
A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample....
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Published in | Analyst (London) Vol. 142; no. 14; pp. 2552 - 2557 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
21.07.2017
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Online Access | Get full text |
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Summary: | A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10
mol) for a peptide standard. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2654 1364-5528 |
DOI: | 10.1039/c7an00544j |