Can hypoosmotic shock and calcium influx lead to translocation of aquaporin‐1 in shrimp muscle cells?

• Published in: Cell biology international Vol. 46; no. 6; pp. 976 - 985
• Main Authors: Foguesatto, Kamila, Lopes, Fernanda Moreira, Boyle, Robert Tew, Nery, Luiz Eduardo Maia, Souza, Marta Marques
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.06.2022
• Subjects: Aquaporin 1; Aquaporins; Calcium (extracellular); Calcium (intracellular); Calcium influx; calcium signaling; Cell membranes; Cell size; crustacean molting; Hemolymph; Homeostasis; hypoosmotic medium; Immunohistochemistry; Intracellular; Molting; regulation; water channels; Water flow; Water uptake; regulation; hypoosmotic medium; crustacean molting; calcium signaling; water channels
• ISSN: 1065-6995; 1095-8355
• DOI: 10.1002/cbin.11788

The physiological variations during the crustacean molting cycle have intrigued researchers for many years. Maintaining osmotic homeostasis in the face of hemolymph dilution and dealing with dynamic intracellular and extracellular calcium fluctuations are challenges these animals continuously confront. It has recently been shown that water channels present in the cell membrane (aquaporins) are essential for water uptake during premolt and postmolt. This study aims to investigate whether hypoosmotic shock and intracellular and extracellular calcium variations can lead to translocation of Aquaporin 1 (AQP‐1) from the intracellular region to the plasma membrane during premolt and postmolt, thus allowing increased water flow in these stages. For this, we investigate in vitro the rapid change of AQP‐1 positions in the abdominal muscle cells in the freshwater shrimp, Palaemon argentinus. Using cell volume analysis and immunohistochemistry, we show that hypoosmotic conditions and an elevation of the intracellular and extracellular calcium concentrations are concurrent with the translocation of AQP‐1 to the plasma membrane. These results indicate that calcium flux and hypoosmotic shock may be regulators of AQP 1 in the translocation process.