Development of a novel HPLC‐ESI‐MS/MS method to analyze the stereoselective pharmacokinetics and tissue distribution of isoconazole enantiomers in rats

• Published in: Chirality (New York, N.Y.) Vol. 34; no. 6; pp. 901 - 912
• Main Authors: Zhu, Yuanyuan, Wang, Lina, Guo, Guoxian, Tang, Jing, Yu, Jia
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2022
• Subjects: Absolute configuration; Acetic acid; Acetonitrile; Ammonium; Ammonium acetate; Antiinfectives and antibacterials; Biological properties; Biological samples; Cardiac muscle; Circular dichroism; Dichroism; Electrospraying; Enantiomers; enantioselective; High performance liquid chromatography; HPLC‐ESI‐MS/MS; Imidazole; In vivo methods and tests; Intestine; Ionization; Ions; isoconazole enantiomers; Kidneys; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Muscles; Pharmacokinetics; plasma quantification; Small intestine; Spleen; Stereoselectivity; tissue distribution; Tissues; isoconazole enantiomers; HPLC-ESI-MS/MS; plasma quantification; enantioselective; tissue distribution
• ISSN: 0899-0042; 1520-636X
• DOI: 10.1002/chir.23442

Isoconazole with an asymmetrical carbon is a broad‐spectrum antimicrobial imidazole, but there is still lack of relevant report about the potential enantioselectivity in biological samples. The object of this research was to develop and validate a sensitive and effective high performance liquid chromatography‐electrospray ionization coupled with tandem mass spectrometry (HPLC‐ESI‐MS/MS) method for stereoselective separation and determination of isoconazole enantiomers in Sprague‐Dawley (SD) rat plasma and tissues. The greater enantioseparation of isoconazole enantiomers was obtained on a Chiralpak IC column with a mobile phase consisted of acetonitrile‐10 mM aqueous ammonium acetate (90:10, v/v) under the reversed‐phase mode. Subsequently, the studied compounds and internal standard (IS) were detected on a multiple reaction monitoring (MRM) mode with positive electrospray ionization source. The experimental and theoretical Electronic Circular Dichroism (ECD) spectra were employed to confirm the absolute configuration of isoconazole enantiomers. Eventually, after full method validation, the newly developed method was successfully applied to the study of enantioselectivity in plasma and tissues in SD rats. Results illustrated that the enantioselective differences in plasma were observed for the evidence that the concentrations of S‐(−)‐isoconazole were always higher than R‐(+)‐isomer. In terms of tissue distribution, liver, kidney, lung, spleen, and small intestine were the mainly distributed tissues and then followed by heart and muscle. This is the first study to reveal the stereoselective behavior of isoconazole enantiomers in vivo, which also provides reliable and valuable reference for further elucidating the enantioselective metabolisms of isoconazole enantiomers. A novel chiral HPLC‐ESI‐MS/MS method for quantification of isoconazole enantiomers was developed. The first and second eluates were collected under the optimized HPLC to determine the absolute configuration and elution order of isoconazole enantiomers. After full validation, the method was applied to stereoselective study in rat plasma and tissues.