Antigen-specific B cells in tissues after oral typhoid vaccination

Six human subjects who were to receive elective bowel surgery for a variety of diseases were vaccinated with the oral typhoid vaccine, Ty21a. Intestinal tissue (ileum in two, large intestine in four) removed 7-26 days after the first dose of vaccine was examined for the presence and distribution of...

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Bibliographic Details
Published inImmunology and cell biology Vol. 73; no. 1; p. 62
Main Authors Gibson, C E, Penttila, I A, Leong, A S, Zola, H, Labrooy, J T
Format Journal Article
LanguageEnglish
Published United States 01.02.1995
Subjects
Administration, Oral
Adult
Aged
Aged, 80 and over
B-Lymphocytes - immunology
Female
Humans
Intestines - immunology
Intestines - pathology
Male
Microscopy, Fluorescence
Middle Aged
Typhoid-Paratyphoid Vaccines - administration & dosage
Typhoid-Paratyphoid Vaccines - immunology
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Summary:Six human subjects who were to receive elective bowel surgery for a variety of diseases were vaccinated with the oral typhoid vaccine, Ty21a. Intestinal tissue (ileum in two, large intestine in four) removed 7-26 days after the first dose of vaccine was examined for the presence and distribution of antigen-specific B cells. This was compared with intestinal tissue derived from two unvaccinated controls. A number of B cell differentiation antigens were also assessed on these cells by immunofluorescence using dual-labelling. Antigen-specific cells were found randomly distributed in the lamina propria of all the vaccinated subjects in low frequency (6 +/- 0.5 to 37 +/- 31 [mean +/- s.e.m.] antigen specific cells/10 mm2 of tissue). The lymphocyte differentiation antigens CD45RA, CD45RO, L-selectin, CD-11a CD-38, CD-44 and VLA-4 were all found on antigen-specific cells, but no particular pattern was recognizable in this small series of six subjects with different disease processes affecting the intestine.
ISSN:0818-9641
DOI:10.1038/icb.1995.10