"Survival" of Mitochondria in vitro: Physical and Energy Parameters

Isolated mitochondria have been maintained active and coupled for 72 hours at 25 C. Survival (retention of respiratory control) is a function of incubation temperature and dependent upon aeration and substrate. ATP does not entirely substitute for substrate, indicating a need for products of active...

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Published inPlant physiology (Bethesda) Vol. 51; no. 4; pp. 702 - 707
Main Authors Romani, Roger J., Sozer Ozelkok
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.04.1973
Subjects
Aeration
Centrifugation
Chemical suspensions
Chloroplasts
Energy
Mitochondria
Organelles
Oxygen
Pelleted feed
Phosphates
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Summary:Isolated mitochondria have been maintained active and coupled for 72 hours at 25 C. Survival (retention of respiratory control) is a function of incubation temperature and dependent upon aeration and substrate. ATP does not entirely substitute for substrate, indicating a need for products of active metabolism other than energy. An improvement in respiratory control is often observed during the first several hours of incubation. Sedimentation and resuspension at 24-hour intervals prolonged survival. As revealed by electron microscopy, mitochondria maintained their basic structure during a 72-hour period at 25 C. Survival is a dynamic, energy-requiring process and must be distinguished from so-called "aging" of organelles at ice temperatures. As a manifestation of partial autonomy, survival may prove useful in assessing aspects of mitochondrial function and the mitochondrial-cellular interrelationship.
Bibliography:ObjectType-Article-1
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ISSN:0032-0889
1532-2548
DOI:10.1104/pp.51.4.702