Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro

• Published in: Neural regeneration research Vol. 7; no. 22; pp. 1688 - 1694
• Main Authors: Lu, Jiang, Lu, Kehuan, Li, Dongsheng
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 05.08.2012; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China; Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China%State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China%Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China; Medknow Publications & Media Pvt Ltd
• Subjects: Apoptosis; Females; Fibroblasts; Growth factors; Hedgehog; Laboratory animals; Nervous system; Physical growth; Proteins; Research and Report: Stem Cells and Neural Regeneration; Stem cells; 体外分化; 信号通路; 分泌模式; 成纤维细胞生长因子受体; 昆明种小鼠; 祖细胞; 神经干; United States > US; China; differentiation; neural regeneration; secretion; neural stem cells; Sonic Hedgehog; dynamic; signal pathway; fibroblast growth factor 8; neural progenitor cells; neurons
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.22.002

In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.