Protective effect of insulin and glucose at different concentrations on penicillin-induced astrocyte death on the primer astroglial cell line

• Published in: Neural regeneration research Vol. 7; no. 24; pp. 1895 - 1899
• Main Author: Mehmet Bülent zdemir Hakan Aka agdas Erdogan Onur Tokgün Aydin Demiray Fenki Semin Cem Becerir
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 25.08.2012; Department of Anatomy, Pamukkale University School of Medicine, Denizli 20070, Turkey%Department of Medical Biology, Pamukkale University School of Medicine, Denizli 20070, Turkey%Department of Neurology, Pamukkale University School of Medicine, Denizli 20070, Turkey%Department of Endocrinology, Turkish Health Ministry, Denizli Hospital, Denizli 20070, Turkey%Department of Pediatrics, Pamukkale University School of Medicine, Denizli 20070, Turkey; Medknow Publications & Media Pvt Ltd
• Subjects: Antibiotics; Apoptosis; Blood-brain barrier; Cell culture; Epilepsy; Glucose; Hyperglycemia; Insulin; Ischemia; Metabolism; Nervous system; Patients; Penicillin; Potassium; Spinal cord; Techniques and Methods: Emerging Technology in Neural Regeneration; Traumatic brain injury; 保护作用; 星形胶质细胞; 浓度; 细胞株; 细胞死亡; 胰岛素受体; 葡萄糖; 青霉素; penicillin; glucose; cell survival; cell death; insulin; rat; cell culture; newborn; brain; astrocyte
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.24.008

Astrocytes perform many functions in the brain and spinal cord.Glucose metabolism is important for astroglial cells and astrocytes are the only cells with insulin receptors in the brain.The common antibiotic penicillin is also a chemical agent that causes degenerative effect on neuronal cell.The aim of this study is to show the effect of insulin and glucose at different concentrations on the astrocyte death induced by penicillin on primer astroglial cell line.It is well known that intracranial penicillin treatment causes neuronal cell death and it is used for experimental epilepsy model commonly.Previous studies showed that insulin and glucose might protect neuronal cell in case of proper concentrations.But,the present study is about the effect of insulin and glucose against astrocyte death induced by penicillin.For this purpose,newborn rat brain was extracted and then mechanically dissociated to astroglial cell suspension and finally grown in culture medium.Clutters were maintained for 2 weeks prior to being used in these experiments.Different concentrations of insulin（0,1,3 nM）and glucose（0,3,30 mM）were used in media without penicillin and with 2 500μM penicillin.Penicillin decreased the viability of astroglial cell seriously.The highest cell viability appeared in medium with 3 nM insulin and 3 mM glucose but without penicillin.However,in medium with penicillin,the best cell survival was in medium with 1 nM insulin but without glucose. We concluded that insulin and glucose show protective effects on the damage induced by penicillin to primer astroglial cell line.Interestingly,cell survival depends on concentrations of insulin and glucose strongly.The results of this study will help to explain cerebrovascular pathologies parallel to insulin and glucose conditions of patient after intracranial injuries.