Site specific chemoselective labelling of proteins with robust and highly sensitive Ru(II) bathophenanthroline complexes

The bioorthogonal and chemoselective fluorescence labelling of several cell-free synthesized proteins containing a site-specifically incorporated azido amino acid was possible using different alkyne-functionalized Ru(II) bathophenanthroline complexes. We were able to achieve a selective labelling ev...

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Published inOrganic & biomolecular chemistry Vol. 10; no. 11; pp. 2223 - 2226
Main Authors Uzagare, Matthew C, Claussnitzer, Iris, Gerrits, Michael, Bannwarth, Willi
Format Journal Article
LanguageEnglish
Published England 21.03.2012
Subjects
Molecular Structure
Phenanthrolines - chemistry
Proteins - analysis
Proteins - chemistry
Ruthenium - chemistry
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Summary:The bioorthogonal and chemoselective fluorescence labelling of several cell-free synthesized proteins containing a site-specifically incorporated azido amino acid was possible using different alkyne-functionalized Ru(II) bathophenanthroline complexes. We were able to achieve a selective labelling even in complex mixtures of proteins despite the fact that ruthenium dyes normally show a high tendency for unspecific interactions with proteins and are commonly used for total staining of proteins. Since the employed Ru complexes are extremely robust, photo-stable and highly sensitive, the approach should be applicable to the production of labelled proteins for single molecule spectroscopy and fluorescence-based interaction studies.
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ISSN:1477-0520
1477-0539
DOI:10.1039/c2ob06684j