Optical Visualization of Cadmium Ion Using a Label-Free Nucleic Acid Aptamer for Recognition

Aptamer-based biosensors have been demonstrated to provide real-time physical and chemical signals for biomedicine, food safety, drug identification, and metal ion determination. In this study, cadmium ion (Cd 2+ ) was determined in drinking water by color signaling using the specific binding of the...

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Bibliographic Details
Published inAnalytical letters Vol. 57; no. 6; pp. 981 - 995
Main Authors Zhang, Qing, Zhang, Chunrong, Li, Nan, Dai, Zhao
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 12.04.2024
Taylor & Francis Ltd
Subjects
Aptamer-based biosensors
Biosensors
Cadmium
cadmium ion
colorimetry
Discoloration
Drinking water
Gold
gold nanoparticles (AuNPs)
Labels
Nitrophenol
Nucleic acids
spectrophotometry
visual detection
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Summary:Aptamer-based biosensors have been demonstrated to provide real-time physical and chemical signals for biomedicine, food safety, drug identification, and metal ion determination. In this study, cadmium ion (Cd 2+ ) was determined in drinking water by color signaling using the specific binding of the aptamer and target by its regulation of the catalytic capacity of gold nanoparticles (AuNPs). When sodium borohydride (NaBH 4 ) and a probe with a catalytic function were present, the solution containing 4-nitrophenol changed from yellow to colorless. Under optimal conditions, the naked eye was sufficient to determine Cd 2+ with a limit of detection (LOD) of 5 μM. The discoloration time showed a positive correlation with Cd 2+ concentration up to 30 μM. This method was label-free, efficient, rapid, and selective for Cd 2+ in water without expensive instruments, suggesting practical applications.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719.2023.2235623