Cytotoxic Potential of Phenolic Glycosides from Stipagrostis plumosa

• Published in: Revista brasileira de farmacognosia Vol. 31; no. 6; pp. 842 - 847
• Main Authors: Bawazeer, Majed, Orabi, Mohamed A. A., Yaseen, Mohammed, Abdelkader, Mohamed Salaheldin A.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2021
• Subjects: Medicine; Pharmacy; Short Communication; Flavonoids; Monocotyledon; Cell lysis; Phenolic glycosides; Proliferation assay; F-actin filament disruption
• ISSN: 1981-528X; 1981-528X
• DOI: 10.1007/s43450-021-00206-w

Chemical analysis of the aqueous and n -butanol fractions of methanolic extract of Stipagrostis plumosa Munro ex T.Anderson, Poaceae, aerial parts led to isolation of seven known phenolic glycosides 3,4′-dihydroxypropiophenone-3- O -β- d -glucoside, 5′-methoxy-3,4′-dihydroxypropiophenone-3- O -β- d -glucoside, 3′5′-dimethoxy-3,4′-dihydroxypropiophenone-3- O -β- d -glucoside, 3,4,5-trimethoxyphenyl-β- d -glucopyranose, tricin-7- O -rutinoside, tricin-7- O -β- d -glucoside, and apigenin-6- C -α- l -arabinopyranosyl-8- C -β- d -glucopyranoside. The cytotoxic activity of the isolates, expressed as IC 50 , was assessed against five different tumor cell lines (JEG-3, HeLa, MCF-7, NCI-H295R, and Hep-G2), cancer like cells (MCF-10A), and normal cells (HDFa) using tamoxifen as a reference drug. All tested compounds exhibited promising activity against JEG-3 cell line as compared to the reference tamoxifen. Fluorescence imaging study indicated the disruption of cell nucleus and lower integrity of F-actin filament, suggesting a potential two-phase cytotoxicity mechanism. Graphical abstract