Efficiency of various strategies to induce triploidy in Mytilus edulis in relation to their first-stage performance

Recently, there is a greater interest for the development of mussel Mytilus edulis hatcheries, particularly for the production and commercialization of triploid bivalves. The aims of this study were to examine the efficiency of inducing triploid mussels by various treatments and to determine the eff...

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Bibliographic Details
Published inJournal of applied aquaculture Vol. 35; no. 2; pp. 473 - 488
Main Authors Osterheld, Kevin, Davidson, John, Comeau, Luc A., Hori, Tiago, Belzile, Claude, Tremblay, Réjean
Format Journal Article
LanguageEnglish
Published Philadelphia Taylor & Francis 03.04.2023
Taylor & Francis Ltd
Subjects
Cold shock
Commercialization
Developmental stages
Diploids
Diploidy
Flow cytometry
growth
Hatcheries
Larvae
Larval development
Larval stage
Marine molluscs
Marketing
Mollusks
mussel farming
Mussels
Mytilus edulis
Polyploidy
Purines
Shock
Survival
Triploidy
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Summary:Recently, there is a greater interest for the development of mussel Mytilus edulis hatcheries, particularly for the production and commercialization of triploid bivalves. The aims of this study were to examine the efficiency of inducing triploid mussels by various treatments and to determine the effect of triploidy on larval development. To produce triploids, extrusion of the second polar body of fertilized eggs was inhibited by 6-(dimethylamino)purine (6-DMAP), cold-shock, or heat-shock treatments. Triploidy was detected by flow cytometry for all the treatments. Treatment with 6-DMAP was the most efficient with a rate of triploidy of 90%, followed by heat-shock (56%) and cold-shock (30%) treatments. No survival differences were observed between diploids and 6-DMAP-induced triploid larvae from the D-shape stage up to their settlement. Moreover, increased growth was observed in triploid larvae compared to diploid larvae. Overall, this study showed that 6-DMAP is a highly efficient method to induce triploidy in mussels, with triploid larvae having faster growth compared to diploid.
ISSN:1045-4438
1545-0805
DOI:10.1080/10454438.2021.1985032