Activity of nucleoside triphosphate diphosphohydrolase, 5′-nucleotidase, and adenosine deaminase in cattle fed on pastures treated with different nitrogen fertilizers

• Published in: Toxicological and environmental chemistry Vol. 99; no. 5-6; pp. 966 - 974
• Main Authors: Favero, Juscivete Fátima, Christ, Ricardo, Gabriel, Mateus Eloir, Henker, Luan Cleber, Stedille, Fernanda Agustini, Piva, Manoela Marchezan, Bottari, Nathieli Bianchin, Schetinger, Maria Rosa Chitolina, Morsch, Vera Maria, Baldissera, Matheus Dellaméa, da Silva, Antonio Waldimir Leopoldino, Mendes, Ricardo Evandro, Da Silva, Aleksandro Schafer
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 03.07.2017; Taylor & Francis Ltd
• Subjects: ADA; Adenosine deaminase; Adenosine diphosphate; Adenosine triphosphate; Agrochemicals; Animals; ATP; Biochemistry; Cattle; Chips; coagulation disorders; ecto-nucleotidases; Enzymes; Feeding; Fertilizers; Intoxication; nitrite; Nitrites; Nitrogen; Nucleotidase; Pasture; Pastures; Platelets; Urea
• ISSN: 0277-2248; 1029-0486
• DOI: 10.1080/02772248.2017.1324859

The aim of this study was to evaluate nucleoside triphosphate diphosphohydrolase, 5′-nucleotidase, and adenosine deaminase activities in cattle fed on cuttings from pastures fertilized with urea compared to animals fed on cuttings from organically fertilized or from non-fertilized pastures. Blood samples were collected up to 9 h after feeding, and nitrite and enzyme activities were evaluated. The animals feeding on urea-fertilized pasture suffered subclinical intoxication. Serum nitrite levels increased by about 50% and adenosine deaminase activity increased almost sixfold 4 and 6 h after feeding on urea-fertilized pasture, and adenosine deaminase in platelets almost doubled 6 and 9 h after feeding. In platelets, nucleoside triphosphate diphosphohydrolase activity with adenosine triphosphate as substrate increased 2, 4, 6 and 9 h after feeding while nucleoside triphosphate diphosphohydrolase with adenosine diphosphate as substrate and 5′-nucleotidase activities increased only 9 h after feeding. Increased activities of these enzymes may be implicated in the pathophysiology of subclinical intoxication since they are involved in many biochemical functions.