Glycogen synthase kinase-3β positively regulates the proliferation of human ovarian cancer cells

• Published in: Cell research Vol. 16; no. 7; pp. 671 - 677
• Main Authors: Cao, Qi, Lu, Xin, Feng, You-Ji
• Format: Journal Article
• Language: English
• Published: 01.07.2006
• Subjects: 卵巢肿瘤; 糖原合酶激酶-3β; 肿瘤细胞; 酶活性
• ISSN: 1001-0602; 1748-7838
• DOI: 10.1038/sj.cr.7310078

Although glycogen synthase kinase-3 （GSK-3） might act as a tumor suppressor since its inhibition is expected to mimic the activation of Wnt-signaling pathway, GSK-3β may contribute to NF-κB activation in cancer cells leading to increased cancer cell proliferation and survival. Here we report that GSK-3β activity was involved in the proliferation of human ovarian cancer cell both in vitro and in vivo. Inhibition of GSK-3 activity by pharmacological inhibitors suppressed proliferation of the ovarian cancer cells. Overexpressing constitutively active form of GSK-3β induced entry into the S phase, increased cyclin D1 expression and facilitated the proliferation of ovarian cancer cells. Furthermore, GSK-3 inhibition prevented the formation of the tumor in nude mice generated by the inoculation of human ovarian cancer cells. Our findings thus suggest that GSK-3β activity is important for the proliferation of ovarian cancer cells, implicating this kinase as a potential therapeutic target in ovarian cancer.