A 3D mammalian cell separator biochip

The dissimilar cytoskeletal architecture in diverse cell types induces a difference in their deformability that presents a viable approach to separate cells in a non-invasive manner. We report on the design and fabrication of a robust and scalable device capable of separating a heterogeneous populat...

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Bibliographic Details
Published inLab on a chip Vol. 12; no. 5; pp. 948 - 953
Main Authors Choudhury, Debaditya, Ramsay, William T, Kiss, Robert, Willoughby, Nicholas A, Paterson, Lynn, Kar, Ajoy K
Format Journal Article
LanguageEnglish
Published England 07.03.2012
Subjects
Animals
Cell Separation
Flow Cytometry
HL-60 Cells
Humans
Lasers
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
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Summary:The dissimilar cytoskeletal architecture in diverse cell types induces a difference in their deformability that presents a viable approach to separate cells in a non-invasive manner. We report on the design and fabrication of a robust and scalable device capable of separating a heterogeneous population of cells with variable degree of deformability into enriched populations with deformability above a certain threshold. The three dimensional device was fabricated in fused silica by femtosecond laser direct writing combined with selective chemical etching. The separator device was evaluated using promyelocytic HL60 cells. Using flow rates as large as 167 μL min(-1), throughputs of up to 2800 cells min(-1) were achieved at the device output. A fluorescence-activated cell sorting (FACS) viability analysis on the cells revealed 81% of the population maintain cellular integrity after passage through the device.
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ISSN:1473-0197
1473-0189
DOI:10.1039/c2lc20939j