Chemical and Enzymatic Synthesis of DNA Fragments Containing 5-(β-D-Glucopyranosyloxymethyl)-2′-deoxycytidine − a Modified Nucleoside in T4 Phage DNA
DNA fragments 23 and 25, containing 5‐hydroxymethyl‐2′‐deoxycytidine (5‐HMdC) and 5‐(β‐D‐glucopyranosyloxymethyl)‐2′‐deoxycytidine (5‐GlcMdC) units, respectively, were assembled by means of a solid support‐assisted synthesis using phosphoramidite building blocks 4 and 5, respectively. In addition, 2...
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Published in | European journal of organic chemistry Vol. 2001; no. 11; pp. 2075 - 2082 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag GmbH
01.06.2001
WILEY‐VCH Verlag GmbH Wiley |
Subjects | |
Online Access | Get full text |
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Summary: | DNA fragments 23 and 25, containing 5‐hydroxymethyl‐2′‐deoxycytidine (5‐HMdC) and 5‐(β‐D‐glucopyranosyloxymethyl)‐2′‐deoxycytidine (5‐GlcMdC) units, respectively, were assembled by means of a solid support‐assisted synthesis using phosphoramidite building blocks 4 and 5, respectively. In addition, 20‐mer 23 was converted into fragment 26 by a glycosylation with uridine diphosphoglucose (UDPG), catalyzed by T4 β‐glucosyltransferase (BGT). |
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Bibliography: | istex:112F1660B40C603ED5A8730801BEB1A516BEAAB6 ArticleID:EJOC2075 ark:/67375/WNG-T81RBSFJ-T |
ISSN: | 1434-193X 1099-0690 |
DOI: | 10.1002/1099-0690(200106)2001:11<2075::AID-EJOC2075>3.0.CO;2-J |