Anti-tumor necrosis factor receptor and tumor necrosis factor agonist activity by an anti-idiotypic antibody

• Published in: European journal of immunology Vol. 22; no. 11; p. 3045
• Main Authors: Galloway, C J, Madanat, M S, Sarr, T, Espevik, T, Dumas, M L, Mitra, G, Ranges, G E
• Format: Journal Article
• Language: English
• Published: Germany 01.11.1992
• Subjects: Animals; Antibodies, Anti-Idiotypic - physiology; Humans; NF-kappa B - metabolism; Rabbits; Receptors, Cell Surface - immunology; Receptors, Tumor Necrosis Factor; Tumor Necrosis Factor-alpha - physiology
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830221143

Tumor necrosis factor (TNF) is a cytokine which, among other properties, is a principle mediator of inflammation and septic shock. It acts upon target cells by binding to specific cell surface receptors. A10G10 is a murine monoclonal antibody which recognizes human TNF and neutralizes its activity. A rabbit polyclonal antibody directed at the antigen-binding site of A10G10 was raised and affinity purified over an A10G10 column. The resultant anti-idiotypic antibody recognized not only A10G10 but also both TNF receptors. It showed TNF agonist activity in two different TNF bioassays, and competed with several anti-TNF receptor monoclonal antibodies and TNF itself for binding to cells. These results represent an example of a method for obtaining antibodies to a ligand-specific receptor in the absence of the receptor itself.