Influenza: propagation, quantification, and storage

Influenza viruses are negative-sense, single-stranded, enveloped RNA viruses belonging to the family Orthomyxoviridae. Three types exist, influenza A, B, and C. All infect humans, but only A and B are major human pathogens. Influenza type A viruses are divided into subtypes based on genetic and anti...

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Bibliographic Details
Published inCurrent protocols in microbiology (Online) Vol. Chapter 15; p. 15G.1.1
Main Authors Balish, Amanda L, Katz, Jacqueline M, Klimov, Alexander I
Format Journal Article
LanguageEnglish
Published United States 2013
Subjects
Animals
Cell Line
Chick Embryo
Dogs
Humans
Influenza, Human - diagnosis
Influenza, Human - virology
Orthomyxoviridae - growth & development
Orthomyxoviridae - isolation & purification
Preservation, Biological
Viral Load
Online AccessGet more information

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Summary:Influenza viruses are negative-sense, single-stranded, enveloped RNA viruses belonging to the family Orthomyxoviridae. Three types exist, influenza A, B, and C. All infect humans, but only A and B are major human pathogens. Influenza type A viruses are divided into subtypes based on genetic and antigenic differences in the two surface spike proteins, hemagglutinin (HA) and neuraminidase (NA). The appropriate cell lines to be used for isolation of influenza A or B viruses depend on the clinical information and the host of origin. MDCK cells are the preferred cell line for isolation of human influenza viruses from clinical specimens.
ISSN:1934-8533
DOI:10.1002/9780471729259.mc15g01s29