Immunoaffinity, capillary electrophoresis, and statistics for studying intact alpha 1-acid glycoprotein isoforms as an atherothrombosis biomarker

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 919; p. 215
• Main Authors: Puerta, Angel, Martin-Alvarez, Pedro J, Ongay, Sara, Diez-Masa, Jose C, de Frutos, Mercedes
• Format: Journal Article
• Language: English
• Published: United States 2013
• Subjects: Atherosclerosis - diagnosis; Biomarkers - metabolism; Discriminant Analysis; Electrophoresis, Capillary - methods; Humans; Immunochromatography - methods; Orosomucoid - isolation & purification; Orosomucoid - metabolism; Protein Isoforms - metabolism; Reference Standards; Statistics as Topic; Thrombosis - diagnosis
• ISSN: 1940-6029
• DOI: 10.1007/978-1-62703-029-8_20

Variations in the amino acid sequence, glycosylation, and/or other posttranslational modifications in glycoproteins give rise to different molecules of the glycoprotein called forms. Qualitative and/or quantitative alterations in these forms are related to pathophysiological situations in the individuals. In this study, a methodology to analyze these differences in forms of the alpha 1-acid glycoprotein (AGP) between healthy individuals and patients with two different vascular diseases is detailed. The whole methodology includes a sample preparation method based on immunochromatography, a capillary electrophoresis method for separation of AGP peaks (isoforms), and statistical methods (Linear Discriminant Analysis) for sample classification. As a result, it is shown that the methodology proposed allows studying the role of AGP isoforms as potential vascular disease biomarkers.