An in vitro system for the quantitative measurement of chitin synthesis in the cockroach: Inhibition by TH 6040 and polyoxin D
The β-ecdysone-dependent in vitro synthesis of chitin by cockroach leg regenerates was confirmed by chemical analysis and provides a sensitive, quantitative bioassay for inhibitors of chitin synthesis or materials with molting hormone activity via 14C-N-acetylglucosamine incorporation. Chitin synthe...
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| Published in | Insect biochemistry Vol. 5; no. 6; pp. 855 - 859 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
01.12.1975
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The β-ecdysone-dependent
in vitro synthesis of chitin by cockroach leg regenerates was confirmed by chemical analysis and provides a sensitive, quantitative bioassay for inhibitors of chitin synthesis or materials with molting hormone activity
via
14C-N-acetylglucosamine incorporation. Chitin synthesis in insect tissue was inhibited (I
50) by TH 6040 at 6·11 × 10
−10 M and by polyoxin D at 7·53 × 10
−7 M. An investigation of the low I
50 of TH 6040 showed that it partitions out of culture medium into tissue. This suggests that this compound may be magnified in food chains. |
|---|---|
| AbstractList | The β-ecdysone-dependent
in vitro synthesis of chitin by cockroach leg regenerates was confirmed by chemical analysis and provides a sensitive, quantitative bioassay for inhibitors of chitin synthesis or materials with molting hormone activity
via
14C-N-acetylglucosamine incorporation. Chitin synthesis in insect tissue was inhibited (I
50) by TH 6040 at 6·11 × 10
−10 M and by polyoxin D at 7·53 × 10
−7 M. An investigation of the low I
50 of TH 6040 showed that it partitions out of culture medium into tissue. This suggests that this compound may be magnified in food chains. |
| Author | Marks, E.P. Sowa, B.A. |
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| Cites_doi | 10.1126/science.167.3914.61 10.7164/antibiotics.27.260 10.2307/1540232 10.1021/jf60199a033 10.1016/0016-6480(73)90107-X 10.1016/0003-2697(60)90025-7 10.2307/1540027 |
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| Keywords | insect polyoxin D TH 6040 chitin synthesis in vitro |
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| References | Bray (BIB1) 1960; 1 Marks, Sowa (BIB7) 1974 Marks, Leopold (BIB6) 1971; 140 Oehler, Holman (BIB8) 1975 Marks (BIB4) 1973; 24 Marks, Leopold (BIB5) 1970; 167 Steel, Torrie (BIB10) 1960 Marks (BIB3) 1972; 142 Post, Mulder (BIB9) 1974 Hori, Eguchi, Kakiki (BIB2) 1974; 27 Marks (10.1016/0020-1790(75)90030-X_BIB6) 1971; 140 Marks (10.1016/0020-1790(75)90030-X_BIB5) 1970; 167 Oehler (10.1016/0020-1790(75)90030-X_BIB8) 1975 Marks (10.1016/0020-1790(75)90030-X_BIB3) 1972; 142 Marks (10.1016/0020-1790(75)90030-X_BIB4) 1973; 24 Post (10.1016/0020-1790(75)90030-X_BIB9) 1974 Hori (10.1016/0020-1790(75)90030-X_BIB2) 1974; 27 Steel (10.1016/0020-1790(75)90030-X_BIB10) 1960 Marks (10.1016/0020-1790(75)90030-X_BIB7) 1974 Bray (10.1016/0020-1790(75)90030-X_BIB1) 1960; 1 |
| References_xml | – volume: 27 start-page: 260 year: 1974 end-page: 266 ident: BIB2 article-title: Studies on the mode of action of polyoxins. VI. Effects of polyoxin B on chitin synthesis in polyoxin-sensitive and resistant strains of publication-title: J. Antibiotics contributor: fullname: Kakiki – volume: 167 start-page: 61 year: 1970 end-page: 62 ident: BIB5 article-title: Cockroach leg regeneration: Effects of ecdysterone in vitro publication-title: Science, Wash. contributor: fullname: Leopold – year: 1975 ident: BIB8 article-title: Residue of Thompson-Hayward 6040 in bovine manure by high performance liquid chromatograph publication-title: J. agric. Fd Chem. contributor: fullname: Holman – volume: 1 start-page: 279 year: 1960 end-page: 287 ident: BIB1 article-title: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter publication-title: Anal. Biochem. contributor: fullname: Bray – start-page: 136 year: 1974 end-page: 143 ident: BIB9 article-title: Insecticidal properties and mode of action of 1-(2,6-dihalogenbenzoyl)-3-phenyl-ureas publication-title: Mechanism of Pesticide Action contributor: fullname: Mulder – volume: 24 start-page: 472 year: 1973 end-page: 477 ident: BIB4 article-title: Deposition of insect cuticle in vitro: Differential responses to α- and β-ecdysone publication-title: Gen. comp. Endocr. contributor: fullname: Marks – start-page: 161 year: 1960 end-page: 171 ident: BIB10 publication-title: Principles and Procedures of Statistics contributor: fullname: Torrie – volume: 142 start-page: 293 year: 1972 end-page: 301 ident: BIB3 article-title: Effects of ecdysterone on the deposition of cockroach cuticle in vitro publication-title: Biol. Bull., Woods Hole contributor: fullname: Marks – volume: 140 start-page: 73 year: 1971 end-page: 83 ident: BIB6 article-title: Deposition of cuticular substances in vitro by leg regenerates from the cockroach, publication-title: Biol. Bull., Woods Hole contributor: fullname: Leopold – start-page: 144 year: 1974 end-page: 155 ident: BIB7 article-title: An in vitro model system for the production of insect cuticle publication-title: Mechanism of Pesticide Action contributor: fullname: Sowa – start-page: 136 year: 1974 ident: 10.1016/0020-1790(75)90030-X_BIB9 article-title: Insecticidal properties and mode of action of 1-(2,6-dihalogenbenzoyl)-3-phenyl-ureas contributor: fullname: Post – start-page: 144 year: 1974 ident: 10.1016/0020-1790(75)90030-X_BIB7 article-title: An in vitro model system for the production of insect cuticle contributor: fullname: Marks – start-page: 161 year: 1960 ident: 10.1016/0020-1790(75)90030-X_BIB10 contributor: fullname: Steel – volume: 167 start-page: 61 year: 1970 ident: 10.1016/0020-1790(75)90030-X_BIB5 article-title: Cockroach leg regeneration: Effects of ecdysterone in vitro publication-title: Science, Wash. doi: 10.1126/science.167.3914.61 contributor: fullname: Marks – volume: 27 start-page: 260 year: 1974 ident: 10.1016/0020-1790(75)90030-X_BIB2 article-title: Studies on the mode of action of polyoxins. VI. Effects of polyoxin B on chitin synthesis in polyoxin-sensitive and resistant strains of Alternaria kikuchiana publication-title: J. Antibiotics doi: 10.7164/antibiotics.27.260 contributor: fullname: Hori – volume: 142 start-page: 293 year: 1972 ident: 10.1016/0020-1790(75)90030-X_BIB3 article-title: Effects of ecdysterone on the deposition of cockroach cuticle in vitro publication-title: Biol. Bull., Woods Hole doi: 10.2307/1540232 contributor: fullname: Marks – year: 1975 ident: 10.1016/0020-1790(75)90030-X_BIB8 article-title: Residue of Thompson-Hayward 6040 in bovine manure by high performance liquid chromatograph publication-title: J. agric. Fd Chem. doi: 10.1021/jf60199a033 contributor: fullname: Oehler – volume: 24 start-page: 472 year: 1973 ident: 10.1016/0020-1790(75)90030-X_BIB4 article-title: Deposition of insect cuticle in vitro: Differential responses to α- and β-ecdysone publication-title: Gen. comp. Endocr. doi: 10.1016/0016-6480(73)90107-X contributor: fullname: Marks – volume: 1 start-page: 279 year: 1960 ident: 10.1016/0020-1790(75)90030-X_BIB1 article-title: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter publication-title: Anal. Biochem. doi: 10.1016/0003-2697(60)90025-7 contributor: fullname: Bray – volume: 140 start-page: 73 year: 1971 ident: 10.1016/0020-1790(75)90030-X_BIB6 article-title: Deposition of cuticular substances in vitro by leg regenerates from the cockroach, Leucophaea maderae (F.) publication-title: Biol. Bull., Woods Hole doi: 10.2307/1540027 contributor: fullname: Marks |
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| Title | An in vitro system for the quantitative measurement of chitin synthesis in the cockroach: Inhibition by TH 6040 and polyoxin D |
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