An in vitro system for the quantitative measurement of chitin synthesis in the cockroach: Inhibition by TH 6040 and polyoxin D

The β-ecdysone-dependent in vitro synthesis of chitin by cockroach leg regenerates was confirmed by chemical analysis and provides a sensitive, quantitative bioassay for inhibitors of chitin synthesis or materials with molting hormone activity via 14C-N-acetylglucosamine incorporation. Chitin synthe...

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Bibliographic Details
Published inInsect biochemistry Vol. 5; no. 6; pp. 855 - 859
Main Authors Sowa, B.A., Marks, E.P.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.12.1975
Subjects
insect
polyoxin D
TH 6040
chitin synthesis
in vitro
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Summary:The β-ecdysone-dependent in vitro synthesis of chitin by cockroach leg regenerates was confirmed by chemical analysis and provides a sensitive, quantitative bioassay for inhibitors of chitin synthesis or materials with molting hormone activity via 14C-N-acetylglucosamine incorporation. Chitin synthesis in insect tissue was inhibited (I 50) by TH 6040 at 6·11 × 10 −10 M and by polyoxin D at 7·53 × 10 −7 M. An investigation of the low I 50 of TH 6040 showed that it partitions out of culture medium into tissue. This suggests that this compound may be magnified in food chains.
ISSN:0020-1790
DOI:10.1016/0020-1790(75)90030-X