Picogram-sensitive assay for endotoxin: Gelation of Limulus polyphemus blood cell lysate induced lipopolysaccharides and lipid A from gram-negative bacteria

The original endotoxin assay of Levin et al. ( N. Engl. J. Med., 283 (1970) 1313) has been improved to allow detection of picogram quantities of lipopolysaccharides and free lipid A. The response of this assay to both alkali-treated and untreated lipopolysaccharides and their derivatives parallels t...

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Published inBiochimica et biophysica acta. General subjects Vol. 261; no. 1; pp. 284 - 289
Main Authors Thye Yin, E., Galanos, Chris, Kinsky, Stephen, Bradshaw, Ralph A., Wessler, Stanford, Lüderitz, Otto, Sarmiento, Marion E.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.01.1972
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Summary:The original endotoxin assay of Levin et al. ( N. Engl. J. Med., 283 (1970) 1313) has been improved to allow detection of picogram quantities of lipopolysaccharides and free lipid A. The response of this assay to both alkali-treated and untreated lipopolysaccharides and their derivatives parallels the previously demonstrated effect of these substances on guinea pig complement fixation.
ISSN:0304-4165
1872-8006
DOI:10.1016/0304-4165(72)90340-6