A role for upstream binding factor in organizing ribosomal gene chromatin

Human ribosomal genes are located in NORs (nucleolar organizer regions) on the short arms of acrocentric chromosomes. During metaphase, previously active NORs appear as prominent chromosomal features termed secondary constrictions, which are achromatic in chromosome banding and positive in silver st...

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Bibliographic Details
Published inBiochemical Society Symposia no. 73; p. 77
Main Authors Wright, Jane E, Mais, Christine, Prieto, José-Luis, McStay, Brian
Format Journal Article
LanguageEnglish
Published England 2006
Subjects
Binding Sites - genetics
Chromatin - genetics
Chromatin - metabolism
DNA, Ribosomal - genetics
DNA, Ribosomal - metabolism
Humans
Nucleolus Organizer Region - genetics
Nucleolus Organizer Region - metabolism
Pol1 Transcription Initiation Complex Proteins - genetics
Pol1 Transcription Initiation Complex Proteins - metabolism
Protein Binding
Ribosomes - genetics
Ribosomes - metabolism
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Summary:Human ribosomal genes are located in NORs (nucleolar organizer regions) on the short arms of acrocentric chromosomes. During metaphase, previously active NORs appear as prominent chromosomal features termed secondary constrictions, which are achromatic in chromosome banding and positive in silver staining. The architectural RNA polymerase I transcription factor UBF (upstream binding factor) binds extensively across the ribosomal gene repeat throughout the cell cycle. Evidence that UBF underpins NOR structure is provided by an examination of cell lines in which large arrays of a heterologous UBF binding sequences are integrated at ectopic sites on human chromosomes. These arrays efficiently recruit UBF even to sites outside the nucleolus, and during metaphase form novel silver-stainable secondary constrictions, termed pseudo-NORs, that are morphologically similar to NORs.
ISSN:0067-8694
1744-1439
DOI:10.1042/bss0730077