Crystals of a gragment of influenza haemagglutinin in the low pH induced conformation

Fusion of the influenza viral membrane with the membrane of the host cell is preceded by a low pH induced conformation change in the viral haemagglutinin. A fragment, consisting of much of the stem domain of influenza haemagglutinin in the low pH induced conformation, has been crystallized by the va...

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Bibliographic Details
Published inJournal of molecular biology Vol. 236; no. 4; pp. 1262 - 1265
Main Authors Bullough, P.A., Hughson, F.M., Treharne, A.C., Ruigrok, R.W.H., Skehel, J.J., Wiley, D.C.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.03.1994
Subjects
cryocrystallography
crystallization
fusion
haemagglutinin
influenza
influenza
fusion
crystallization
haemagglutinin
cryocrystallography
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Summary:Fusion of the influenza viral membrane with the membrane of the host cell is preceded by a low pH induced conformation change in the viral haemagglutinin. A fragment, consisting of much of the stem domain of influenza haemagglutinin in the low pH induced conformation, has been crystallized by the vapour diffusion method. Several crystal forms have been obtained and the molecular packing in these crystals is discussed. Crystals suitable for the recording of high angle X-ray diffraction data grow in space group C222 1. Diffraction data have been recorded from crystals cooled to −170°C in a cryoprotectant buffer.
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(94)90027-2