Ether-phospholipid composition in neutrophile and platelets

• Published in: Thrombosis research Vol. 38; no. 3; pp. 207 - 214
• Main Authors: Tencé, Martine, Jouvin-Marche, Evelyne, Bessou, Gilles, Record, Michel, Benveniste, Jacques
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.05.1985
• Subjects: ether-lipids; human neutrophils; paf-acether; Rabbit platelets; paf-acether; Hepes, N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid; lyso-paf-acether, non-acetylated paf-acether; EDTA, ethylenediaminotetraacetic acid; Paf-acether, platelet-activating factor; human neutrophils; ether-lipids; Rabbit platelets; TLC, thin-layer chromatography
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/0049-3848(85)90148-3

It is well documented that ether-lipids, especially the 1-O-alkyl-2-O-acyl- sn -glycero-3-phosphocholine, can serve as precursors in paf-acether (platelet-activating factor) biosynthesis. This study was undertaken to determine the amount of these compounds in two cell types which are good producers of paf-acether : human neutrophils and rabbit platelets. The method of phospholipid analysis was based on selective destruction of diacyl molecules by lipase from guinea pig pancreas and of plasmalogens by acidolysis ; phospholipids were then separated by bidimensional thin-layer chromatography. In platelets as in neutrophils, the major phospholipids were choline (37%) and ethanolamine (30 and 32 %, respectively) phosphoglycerides and sphingomyelin (18 and 16%, respectively). In rabbit platelets the 1-alkyl molecules represented 15% of the choline class (0.7 nmol/10 7 cells). Neutrophils differed strikingly from platelets by the high level of these molecules which constituted 50% of the choline class (16.3 nmol/10 7 cells). It therefore appears that in the two cell types, the amount of 1-O-alkyl-2-O-acyl- sn -glycero-3-phosphocholine largely exceeded what would be necessary for paf-acether biosynthesis.