Rice transformation with a senescence-inhibition chimeric gene

A senescence-inhibition chimeric gene containing the specific promoter of SAG12 and IPT gene was transferred into rice with the biolistic method. Results of PCR, Dot blotting and Southern blotting indicated that the chimeric gene had been integrated into rice genome. Analyses of GUS activity and cyt...

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Bibliographic Details
Published inChinese science bulletin Vol. 43; no. 21; pp. 1810 - 1815
Main Authors Fu, Yongcai, Ding, Yueyun, Liu, Xinfang, Sun, Chuanqin, Cao, Souyun, Wang, Dongjiang, He, Sijie, Wang, Xiangkun, Li, Liangcai, Tian, Wenzhong
Format Journal Article
LanguageEnglish
Published Beijing Springer Nature B.V 01.11.1998
Subjects
biolistic
Cytokinins
gene
Genetic transformation
Genomes
IPT
promoter
Rice
Senescence
senescence-inhibition
Southern blotting
specific
transformation
Transgenic plants
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Summary:A senescence-inhibition chimeric gene containing the specific promoter of SAG12 and IPT gene was transferred into rice with the biolistic method. Results of PCR, Dot blotting and Southern blotting indicated that the chimeric gene had been integrated into rice genome. Analyses of GUS activity and cytokinin content in transgenic plants of rice and the observation of T1 generation plant at grain formation stage indicated that the foreign gene was expressed.
Bibliography:FU Yongcai 1, 2, DING Yueyun 1, LIU Xinfang 1, SUN Chuanqin 2, CAO Souyun 1, WANG Dongjiang 1, HE Sijie 1, WANG Xiangkun 2, LI Liangcai 1 and TIAN Wenzhong 1* 1. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, Chin
10-1298/N
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/BF02883378