Specificity and sensitivity of Salmonella typhimurium YG1041 and YG1042 strains possesing elevated levels of both nitroreductase and acetyltransferase activity

Acetyltransferase and nitroreductase are enzymes involved in the intracellular metabolic activation of nitroarenes and/or aromatic amines in Salmonella typhimurium. The plasmid carrying both the acetyltransferase and nitroreductase genes was introduced into S. typhimurium TA98 and TA100. The resulti...

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Published inMutation Research/Environmental Mutagenesis and Related Subjects Vol. 291; no. 3; pp. 171 - 180
Main Authors Hagiwara, Yuji, Watanabe, Masahiko, Oda, Yoshimitsu, Sofuni, Toshio, Nohmi, Takehiko
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.06.1993
Subjects
Acetyltransferase
Aromatic amines
Nitroarenes
Nitroreductase
Salmonella typhimurium
Acetyltransferase
Aromatic amines
Nitroarenes
Salmonella typhimurium
Nitroreductase
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Summary:Acetyltransferase and nitroreductase are enzymes involved in the intracellular metabolic activation of nitroarenes and/or aromatic amines in Salmonella typhimurium. The plasmid carrying both the acetyltransferase and nitroreductase genes was introduced into S. typhimurium TA98 and TA100. The resulting strains, YG1041 and YG1042, respectively, showed high levels of both enzyme activities and were more sensitive to the mutagenic action of some nitro-aromatic compounds such as 2-nitrofluorene, 1-nitropyrene and p-nitrophenetole than did the sensitive strains previously established in this laboratory or the conventional strains. These results indicate that the new strains permit the very efficient detection of the mutagenicity of nitroarenes in the environment.
ISSN:0165-1161
DOI:10.1016/0165-1161(93)90157-U