Identification of the domain within fibroblast growth factor-1 responsible for heparin-dependence

• Published in: Biochimica et biophysica acta. Molecular cell research Vol. 1266; no. 2; pp. 124 - 130
• Main Authors: Imamura, Toru, Friedman, Stanley A., Gamble, Susan, Tokita, Yoshihito, Opalenik, Susan R., Thompson, John A., Maciag, Thomas
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.04.1995
• Subjects: Fibroblast growth factor; Heparin dependence; Fibroblast growth factor; Heparin dependence; ORF; C; FGF; PCR
• ISSN: 0167-4889; 1879-2596
• DOI: 10.1016/0167-4889(95)00009-H

While the prototype members of the fibroblast growth factor (FGF) family, FGF-1 and FGF-2 are structurally related, the structural differences between these polypeptides predict that they will ultimately exhibit different biological roles. Indeed, a significant difference between these proteins is the dependence of FGF-1 on heparin for the generation of maximal mitogenic activity. In order to gain structural insight into the issue of FGF-I heparin-dependence, a synthetic gene encoding FGF-2 was constructed with oligonucleotides in a four-cassette format similar to a synthetic gene previously constructed for FGF-1 (Forough et al. 1992, Biochem. Biophys. Acta 1090 293–298). This strategy permitted the molecular shuffling of corresponding cassette(s) between FGF-1 and FGF-2 to yield FGF-1:FGF-2 chimeras. Three amino acid changes (Lys86 → Glu, Tyr120 → His, and Thr121 → Ala) were introduced into the synthetic FGF-2 gene by the cassette format to generate convenient FGF-I restriction sites, but these alterations did not significantly affect the mitogenic activity or the heparin-binding affinity of the recombinant FGF-2 protein when compared with native FGF-2. Among the various FGF-1:FGF-2 chimeric constructs, one designated FGF-C (1( 1 2 )11) , which represents FGF-1 containing FGF-2 amino acid residues 65 to 81, displayed FGF-1-like heparin-binding affinity but it did not require the addition of exogenous heparin to manifest its mitogenic activity. These data suggest that the sequence within residues 65 and 81 from FGF-2 significantly contributes to the heparin-dependent character of FGF-1.