Inhibitory Effect of 20(S)-Ginsenoside Rg3 on Human Platelet Aggregation and Intracellular Ca2+ Levels via Cyclic Adenosine Monophosphate Dependent Manner
Intracellular Ca 2+ ([Ca 2+ ] i ) induces platelet aggregation, and influences the activation of aggregation associated-molecules. The increased [Ca 2+ ] i activates both the Ca 2+ /calmodulin-dependent phosphorylation of the myosin light chain and the diacylglycerol-dependent phosphorylation of ple...
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Published in | Preventive nutrition and food science Vol. 23; no. 4; pp. 317 - 325 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
한국식품영양과학회
01.12.2018
The Korean Society of Food Science and Nutrition |
Subjects | |
Online Access | Get full text |
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Summary: | Intracellular Ca
2+
([Ca
2+
]
i
) induces platelet aggregation, and influences the activation of aggregation associated-molecules. The increased [Ca
2+
]
i
activates both the Ca
2+
/calmodulin-dependent phosphorylation of the myosin light chain and the diacylglycerol-dependent phosphorylation of pleckstrin to trigger granule secretion (i.e., dense body and α-granule) and platelet aggregation. This study was carried out to elucidate the antagonistic effect of 20(S)-ginsenoside Rg3 (G-Rg3) present in
Panax ginseng
Mayer on Ca
2+
. G-Rg3 inhibited thrombin-induced human platelet aggregation in a dose-dependent manner and suppressed thrombin-induced elevation of [Ca
2+
]
i
mobilization. G-Rg3 increased the levels of cAMP, and subsequently, elevated the phosphorylation of inositol 1,4,5-triphosphate receptor I (Ser
1756
) during thrombin-induced human platelet aggregation. Moreover, G-Rg3 inhibited thapsigargin-induced Ca
2+
influx and the thrombin-induced elevation of extracellular signal-regulated kinase 2 phosphorylation. G-Rg3 exhibited an inhibitory effect on [Ca
2+
]
i
levels leading to granule release and thus a therapeutic potential against platelet-mediated thrombotic disease is suggested. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2287-1098 2287-8602 |
DOI: | 10.3746/pnf.2018.23.4.317 |