Inhibitory Effect of 20(S)-Ginsenoside Rg3 on Human Platelet Aggregation and Intracellular Ca2+ Levels via Cyclic Adenosine Monophosphate Dependent Manner

• Published in: Preventive nutrition and food science Vol. 23; no. 4; pp. 317 - 325
• Main Author: Kwon, Hyuk-Woo
• Format: Journal Article
• Language: English
• Published: 한국식품영양과학회 01.12.2018; The Korean Society of Food Science and Nutrition
• Subjects: 식품과학; p-selectin expression; inositol 1,4,5-triphosphate receptor I; extracellular signal-regulated kinases; 20(S)-ginsenoside Rg3
• ISSN: 2287-1098; 2287-8602
• DOI: 10.3746/pnf.2018.23.4.317

Intracellular Ca 2+ ([Ca 2+ ] i ) induces platelet aggregation, and influences the activation of aggregation associated-molecules. The increased [Ca 2+ ] i activates both the Ca 2+ /calmodulin-dependent phosphorylation of the myosin light chain and the diacylglycerol-dependent phosphorylation of pleckstrin to trigger granule secretion (i.e., dense body and α-granule) and platelet aggregation. This study was carried out to elucidate the antagonistic effect of 20(S)-ginsenoside Rg3 (G-Rg3) present in Panax ginseng Mayer on Ca 2+ . G-Rg3 inhibited thrombin-induced human platelet aggregation in a dose-dependent manner and suppressed thrombin-induced elevation of [Ca 2+ ] i mobilization. G-Rg3 increased the levels of cAMP, and subsequently, elevated the phosphorylation of inositol 1,4,5-triphosphate receptor I (Ser 1756 ) during thrombin-induced human platelet aggregation. Moreover, G-Rg3 inhibited thapsigargin-induced Ca 2+ influx and the thrombin-induced elevation of extracellular signal-regulated kinase 2 phosphorylation. G-Rg3 exhibited an inhibitory effect on [Ca 2+ ] i levels leading to granule release and thus a therapeutic potential against platelet-mediated thrombotic disease is suggested.