Enzyme-linked Immunosorbent Assay for Coronaviruses HCV 229E and MHV 3

Division of Communicable Diseases, Clinical Research Centre, Harrow, Middlesex HA1 3UJ, U.K. The antigenic relationship between human coronavirus strain 229E (HCV 229E) and mouse hepatitis virus strain 3 (MHV 3) was studied by means of the indirect form of enzyme-linked immunosorbent assay (ELISA)....

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Bibliographic Details
Published inJournal of general virology Vol. 49; no. 1; pp. 83 - 89
Main Authors Kraaijeveld, Cornelis A, Madge, M. Hilary, Macnaughton, Malcolm R
Format Journal Article
LanguageEnglish
Published England Soc General Microbiol 01.07.1980
Subjects
Animals
Antigens, Viral - analysis
Cattle - blood
Coronaviridae - immunology
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Immune Sera
Murine hepatitis virus - immunology
Rabbits - blood
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Summary:Division of Communicable Diseases, Clinical Research Centre, Harrow, Middlesex HA1 3UJ, U.K. The antigenic relationship between human coronavirus strain 229E (HCV 229E) and mouse hepatitis virus strain 3 (MHV 3) was studied by means of the indirect form of enzyme-linked immunosorbent assay (ELISA). A cross-reaction was found with hyperimmune rabbit sera between HCV 229E and MHV 3 which may be due to the adherence of bovine serum components from tissue culture media, which were present on virus particles even after extensive purification. No cross-reaction was observed with immune sera absorbed with bovine serum, or with HCV 229E grown in tissue culture without serum. This indirect ELISA with HCV 229E may prove to be useful for studies with human sera. Received 6 November 1979; accepted 1 February 1980.
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ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-49-1-83