Acute hypoxia exposure following prenatal stress impairs hippocampus and novelty‐seeking behavior in adolescent rats

• Published in: International journal of developmental neuroscience Vol. 82; no. 1; pp. 85 - 95
• Main Authors: Çelik, Kübra, Bilim, Petek, Garip, Gurur, Durmaz, Burak, Yildirim Sözmen, Eser, Baka, Meral
• Format: Journal Article
• Language: English
• Published: United States 01.02.2022
• Subjects: Animals; Exploratory Behavior; Female; hippocampus; Hippocampus - metabolism; hypoxia; Hypoxia - metabolism; novelty‐seeking; Pregnancy; Prenatal Exposure Delayed Effects - metabolism; prenatal stress; Rats; Stress, Psychological; Vascular Endothelial Growth Factor A - metabolism; hypoxia; novelty-seeking; prenatal stress; hippocampus
• ISSN: 0736-5748; 1873-474X
• DOI: 10.1002/jdn.10162

Objectives The present study aimed to investigate the effects of acute hypoxia exposure following prenatal stress on the novelty‐seeking behavior and hippocampus of adolescent rats. Methods The offspring were divided into prenatal stress (PS) and non‐stress (NS) groups. Both groups were exposed to hypoxia on postnatal day 10 (P10) while control groups were undisturbed. Novel object recognition task was performed in each group. Next, brains were collected to examine hippocampus via immunohistochemical and biochemical studies on postnatal day 35 (P35). Results PS decreased novelty discrimination and synaptophysin (SYN) expressions in both CA1 and CA3 of the hypoxia group prominently (p < 0.05). Nestin‐expressing cells were reduced while vascular endothelial growth factor (VEGF) expression was enhanced in the subgranular zone (SGZ) of PS‐hypoxia group (p < 0.05). VEGF enhancement triggered angiogenesis in the CA1 and CA3 significantly (p < 0.05). PS also increased thiobarbituric acid reactive substances (TBARS) levels in the hypoxia group as a result of oxidative stress (p < 0.05). Conclusion These findings demonstrated that PS exacerbates neurodevelopmental deficits in the hippocampus of acute hypoxia‐induced offspring in adolescence. The present study showed the profound effects of prenatal stress and neonatal acute hypoxia on hippocampus development and novelty‐seeking behavior of adolescent rats.