A study of the spatial organisation of microbial cells in a gel matrix subjected to treatment with ultrasound standing waves

• Published in: Bioseparation Vol. 10; no. 4-5; pp. 153 - 162
• Main Authors: Gherardini, L, Radel, S, Sielemann, S, Doblhoff-Dier, O, Gröschl, M, Benes, E, McLoughlin, A J
• Format: Journal Article
• Language: English
• Published: Netherlands 2001
• Subjects: Cells, Immobilized; Electronics; Gels; Microscopy, Electron, Scanning; Mycology - methods; Saccharomyces cerevisiae - cytology; Ultrasonics
• ISSN: 0923-179X
• DOI: 10.1023/A:1016311410219

Retention and manipulation of microbial cells through exploitation of ultrasonic forces has been reported as a novel cell immobilisation technique. The spatial ordering of yeast cells, within suspensions subjected to an ultrasonic standing wave field, was analysed for the first time. A technique, based on 'freezing' the spatial arrangement using polymer gelation was developed. The resultant gel was then sectioned and examined using microscopic techniques. Light Microscopy confirmed the presence of specific regions in the ultrasonic field, where the cells are organised into bands corresponding to the standing waves' pressure nodal planes. Computer Image Analysis measurement of several physical parameters associated with this cell distribution matched the values derived from the theoretical model. The spatial cell-cell re-arrangement within each band and uneven distribution along the nodal planes have been analysed by Scanning Electron Microscopy. These results complement the ongoing study of the process of immobilisation of microbial cells by ultrasound standing waves.