effects of paclobutrazol, abscisic acid, and gibberellin on germination and early growth in silver, red, and hybrid maple

• Published in: Canadian journal of forest research Vol. 30; no. 4; pp. 557 - 565
• Main Authors: Marshall, J, Beardmore, T, Whittle, C.A, Wang, B, Rutledge, R.G, Blumwald, E
• Format: Journal Article
• Language: English
• Published: Ottawa, Canada NRC Research Press 01.04.2000; National Research Council of Canada; Canadian Science Publishing NRC Research Press
• Subjects: abscisic acid; Acer rubrum; Acer saccharinum; Agronomy. Soil science and plant productions; Biological and medical sciences; Breeding; Deciduous trees; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Germination; gibberellins; growth; interspecific hybridization; paclobutrazol; seed germination; seeds; Seeds and other planting stocks; storage; Ontario; Acer saccharinum; Growth; Interspecific comparison; Germination; Forest regeneration; Plant juvenile growth stage; Gibberellin; Optimization; Aceraceae; Acer rubrum; Paclobutrazol; Dicotyledones; Sesquiterpenes; Seed; Angiospermae; Triazole derivatives; Abscisic acid; Plant growth substance; Hardwood forest tree; Spermatophyta; Application method; Gibberellic acid; Inhibition
• ISSN: 0045-5067; 1208-6037
• DOI: 10.1139/x99-254

Silver maple (Acer saccharinum L.) seeds were used to examine the mechanisms regulating recalcitrant seed germination aiming towards the development of a biochemical method to temporarily inhibit germination without refrigeration. Treatment of fresh silver maple seeds with the cytochrome p(450) inhibitor paclobutrazol delayed germination and reduced root and shoot extension. The inhibitory effect of paclobutrazol plus abscisic acid (ABA) on the shoots of silver maple could not be overcome by a brief treatment with gibberellic acid A(3) (GA(3)). Red maple seeds (Acer rubrum L.) were almost completely inhibited by the treatment with paclobutrazol alone, and this inhibition could not be overcome by a brief treatment with GA(3). In the red x silver hybrid, root growth was little affected by paclobutrazol plus ABA; however, shoot growth was markedly inhibited by paclobutrazol, and this inhibition was sharply increased by the combination with ABA but partially alleviated by GA(3). Treating fresh silver maple seeds with paclobutrazol for 12 h followed by storage under ideal germination conditions, in the presence of 1 mM ABA, completely prevented germination for 1 week. Root extension was completely restored by the continuous provision of GA(3) along with the ABA.