IDDM in BB rats. Enhanced MHC class I heavy-chain gene expression in pancreatic islets
IDDM in BB rats. Enhanced MHC class I heavy-chain gene expression in pancreatic islets. S J Ono , B Issa-Chergui , E Colle , R D Guttmann , T A Seemayer and A Fuks McGill Cancer Centre, Department of Medicine, McGill University Faculty of Medicine, Montreal, Quebec, Canada. Abstract Modulation in ma...
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Published in | Diabetes (New York, N.Y.) Vol. 37; no. 10; pp. 1411 - 1418 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
American Diabetes Association
01.10.1988
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Online Access | Get full text |
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Summary: | IDDM in BB rats. Enhanced MHC class I heavy-chain gene expression in pancreatic islets.
S J Ono ,
B Issa-Chergui ,
E Colle ,
R D Guttmann ,
T A Seemayer and
A Fuks
McGill Cancer Centre, Department of Medicine, McGill University Faculty of Medicine, Montreal, Quebec, Canada.
Abstract
Modulation in major histocompatibility complex (MHC) gene expression correlates with the inflammatory reactions that occur
during graft rejection and autoimmune disease. We analyzed the expression of class I and II MHC genes in the pancreatic islets
of prediabetic and newly diabetic BB rats by immunohistochemistry of tissue sections and Northern blotting of RNA extracted
from isolated islets. We show that enhanced levels of MHC class I heavy-chain RNA are present in pancreatic islets before
overt inflammation and the onset of insulin-dependent diabetes mellitus (IDDM) in the spontaneously diabetic BB rat. Immunohistochemical
analysis revealed enhanced class I antigen expression throughout the pancreatic islets of newly diabetic animals but no induction
of class II antigen on endocrine cells within the islet. Varying degrees of inflammatory infiltrate were observed in the sections
exhibiting enhanced class I antigen expression or in nearby serial sections. Southern blot analysis revealed no restriction-fragment-length
polymorphism or amplification of the endogenous class I heavy-chain genes compared with those of seroidentical disease-resistant
Wistar-Furth rats. I-A alpha and I-E alpha hybridizing RNA appeared de novo before overt diabetes, although concomitantly
with T-lymphocyte-receptor beta-chain and interferon-gamma gene hybridizing RNA and after MHC class I heavy-chain RNA enhancement
was observed. These data indicate the possibility that enhanced class I heavy-chain gene expression plays a role in the progression
of IDDM. |
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ISSN: | 0012-1797 1939-327X 0012-1797 |
DOI: | 10.2337/diabetes.37.10.1411 |