Integrated analysis of gene expressions and metabolite features unravel immunometabolic interplay in hybrid fish (Carassius cuvieri ♀ × Carassius auratus red var ♂) infected with Aeromonas hydrophila

• Published in: Aquaculture Vol. 563; p. 738981
• Main Authors: Xiong, Ning-Xia, Fang, Zi-Xuan, Kuang, Xu-Ying, Ou, Jie, Luo, Sheng-Wei, Liu, Shao-Jun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.01.2023
• Subjects: Aeromonas hydrophila; antioxidants; aquaculture; Carassius auratus; Carassius cuvieri; Crucian carp; fatty acids; fish; genes; hybrids; hydrolysis; immune response; Immunometabolism; kidneys; metabolites; Metabolome; phospholipids; Transcriptome; Metabolome; Crucian carp; Immunometabolism; Transcriptome
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2022.738981

Aeromonas hydrophila is one of important pathogenic bacteria that can imperil fish survival. Current study suggested that hybrid crucian carp (WR) receiving acute A. hydrophila challenge exerted a molecular regulation linking peroxisomal fatty acid β-oxidation to immune response. A. hydrophila infection may alleviate antioxidant status along with the significant alternation of immune-, apoptosis- and metabolic-related gene expressions in kidney, whereas most portions of differential metabolites (DMs) were enriched in steroid derivatives and phospholipids. In addition, mevalonate (MVA) pathway and phospholipid hydrolysis were identified as crucial cellular processes in WR after A. hydrophila challenge. These results may provide an insight into immunometabolic strategy against A. hydrophila infection. •Acute A. hydrophila infection could induce antioxidant imbalance in WR.•Changes of immune- and metabolic-related gene expressions in kidney were observed.•A. hydrophila infection exhibit a profound effect on MVA pathway and phospholipid hydrolysis.