Colloidal delivery of vitamin E into solid lipid nanoparticles as a potential complement for the adverse effects of anemia treatment

• Published in: Chemistry and physics of lipids Vol. 249; p. 105252
• Main Authors: Gambaro, Rocío C., Berti, Ignacio Rivero, Cacicedo, Maximiliano L., Gehring, Stephan, Alvarez, Vera A., Castro, Guillermo R., Seoane, Analía, Padula, Gisel, Islan, German A.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.11.2022
• Subjects: Anemia; Anemia - chemically induced; Anemia - drug therapy; Antioxidants - pharmacology; Colloids; Drug Carriers; Humans; Iron; Lipids; Nanoparticles; Particle Size; Solid lipid nanoparticles; Vitamin E; Iron; Vitamin E; Anemia; Colloids; Solid lipid nanoparticles
• ISSN: 0009-3084; 1873-2941
• DOI: 10.1016/j.chemphyslip.2022.105252

Vitamin E (VitE) is one of the most important antioxidants and plays a key role in decreasing the inflammatory effects of oxidative stress caused by recurrent doses of iron administration in anemia treatment. However, VitE is poorly soluble in aqueous environments. Here, VitE encapsulation into solid lipid nanoparticles (SLN) composed of myristil myristate to improve its bioavailability was proposed. A 99.9 ± 0.1% encapsulation efficiency with a drug/lipid ratio of 500 µg/mg and 478 higher VitE solubility was obtained. The antioxidant properties of VitE after encapsulation were maintained. SLN-VitE showed a 228.2 nm mean diameter with low polidispersitivity (0.335), and negative Z potential (ζ ≈ −9.0 mV). The SLN were well-dispersed, displayed spherical and homogeneous morphology by TEM. A controlled release of VitE from SLN was found. The XRD and FTIR analyses revealed the presence of a nanostructured architecture of SLN after VitE incorporation. We probed the safety of SLN-VitE after contact with three in vitro cell models: erythrocytes, lymphocytes and HepG2 cells. The cell viability in presence of SLN, SLN-VitE, and their combinations with iron was not affected. The comet assay demonstrated that the DNA damage caused by iron administration was decrease in presence of SLN-VitE. [Display omitted] •Vitamin E (VitE) was successfully encapsulated into lipidic nanoparticles (SLN).•Vit-E modified the crystalline architecture of the SLN.•No hemotoxicity of the formulations was observed.•SLN-VitE did not affect the viability of lymphocytes and HepG2 cell model.•SLN-VitE decrease the genomic damage caused by iron.