Immunoradiometric assay of factor VIII: coagulant antigen using four human antibodies. Study of 27 cases of haemophilia A

Immunoradiometric assay (IRMA) of VIII:C antigen was performed using either IgG or monovalent Fab fragments from four antibodies arisen in polytransfused haemophilia A patients (titre between 100 and 1500 U/ml). Using IgG isolated by a solid or a liquid phase system, only the high titre (greater tha...

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Bibliographic Details
Published inBritish journal of haematology Vol. 47; no. 2; p. 269
Main Authors Girma, J P, Lavergne, J M, Meyer, D, Larrieu, M J
Format Journal Article
LanguageEnglish
Published England 01.02.1981
Subjects
Antibody Affinity
Antigens - analysis
Blood Coagulation
Dose-Response Relationship, Immunologic
Factor VIII - immunology
Hemophilia A - immunology
Humans
Immunoglobulin Fab Fragments - immunology
Immunoglobulin G - immunology
Male
Radioimmunoassay - methods
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Summary:Immunoradiometric assay (IRMA) of VIII:C antigen was performed using either IgG or monovalent Fab fragments from four antibodies arisen in polytransfused haemophilia A patients (titre between 100 and 1500 U/ml). Using IgG isolated by a solid or a liquid phase system, only the high titre (greater than or equal to 1000 U/ml) antibodies could be used for IRMA, with a sensitivity of 0.2% VIII:CAg. Using Fab fragments isolated by liquid phase, high and low (less than or equal to 150 U/ml) titre antibodies could be used and the IRMA was significantly improved with a 10-fold higher sensitivity. The affinity of the antibodies for VIII:CAg, studied by displacement curves using a modification of the IRMA, was found not to depend upon the titre of the antibody. Comparative levels of VIII:C and VIII:CAg in 27 cases of haemophilia A emphasize the heterogeneity of this disorder, two types of severe and three types of mild haemophilia being observed.
ISSN:0007-1048
1365-2141
DOI:10.1111/j.1365-2141.1981.tb02788.x