An Adaptation of the Immunoelectrophoretic Method for the Use of Chicken-Precipitating Antisera

Summary To create optimum conditions to test chicken-precipitating antibody by immunoelectrophoresis, it was necessary to increase the NaCl content of the agar strip. Clear, sharp differentiation of precipitin lines was evident when the agar contained 1.0 to 2.0% NaCl. Lesser amounts failed to preci...

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Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 95; no. 3; pp. 418 - 421
Main Authors Tempelis, C. H, Lofy, Mary F
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.09.1965
Subjects
Agar
Animals
Antibodies
Antigen-Antibody Reactions
Immunodiffusion
Immunoelectrophoresis
In Vitro Techniques
Poultry
Sodium Chloride
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Summary:Summary To create optimum conditions to test chicken-precipitating antibody by immunoelectrophoresis, it was necessary to increase the NaCl content of the agar strip. Clear, sharp differentiation of precipitin lines was evident when the agar contained 1.0 to 2.0% NaCl. Lesser amounts failed to precipitate all antigen-antibody systems and greater amounts produced diffuseness which made interpretation difficult.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.95.3.418