Quantification of a major bovine allergen by a two-site immunometric assay based on monoclonal antibodies

A two-site immunometric assay based on monoclonal antibodies (mAb) was developed for the measurement of a 20-kDa major allergen of cow. The sensitivity of this assay was (BDA20) 1 ng/ml. It was used to measure airborne allergen concentrations in 10 Finnish cowsheds. The mean concentration of the BDA...

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Bibliographic Details
Published inAllergy (Copenhagen) Vol. 49; no. 9; p. 707
Main Authors Ylönen, J, Virtanen, T, Rytkönen, M, Mäntyjärvi, R
Format Journal Article
LanguageEnglish
Published Denmark 01.10.1994
Subjects
Air Pollution, Indoor - analysis
Allergens - analysis
Allergens - immunology
Animals
Antibodies, Monoclonal
Antigen-Antibody Reactions - immunology
Cats
Cattle
Cross Reactions
Dogs
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Horses
Immunization - methods
Mice
Mice, Inbred BALB C
Protein Binding
Sensitivity and Specificity
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Summary:A two-site immunometric assay based on monoclonal antibodies (mAb) was developed for the measurement of a 20-kDa major allergen of cow. The sensitivity of this assay was (BDA20) 1 ng/ml. It was used to measure airborne allergen concentrations in 10 Finnish cowsheds. The mean concentration of the BDA20 measured at two stationary sites was 280 ng/m3. Concentrations varied more than 10-fold among cowsheds (54-804 ng/m3). The mean intertest coefficient of variation was 8.2%, and the mean intratest variation 4.1%.
ISSN:0105-4538
1398-9995
DOI:10.1111/j.1398-9995.1994.tb02091.x