Rapid assay for amino acids in serum or urine by pre-column derivatization and reversed-phase liquid chromatography

This method for estimating clinically important amino acids in serum or urine within 40 min involves o-phthalaldehyde/2-mercaptoethanol derivatization and reversed-phase "high-pressure" liquid chromatography. Homocysteic acid is an internal standard, and homoserine and norvaline are refere...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 28; no. 3; pp. 527 - 531
Main Authors Turnell, DC, Cooper, JD
Format Journal Article
LanguageEnglish
Published England Am Assoc Clin Chem 01.03.1982
Subjects
Amino Acids - analysis
Amino Acids - blood
Amino Acids - urine
Chromatography, High Pressure Liquid - methods
Humans
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Summary:This method for estimating clinically important amino acids in serum or urine within 40 min involves o-phthalaldehyde/2-mercaptoethanol derivatization and reversed-phase "high-pressure" liquid chromatography. Homocysteic acid is an internal standard, and homoserine and norvaline are reference peaks. For all the amino acids estimated, the between-run coefficients of variation ranged from 2.0 to 13.5%, and the mean analytical recoveries from both serum and urine samples was 101%. Peak areas vary linearly with concentration up to 1500 mumol/L for all the amino acids assayed. The limit of detection for each amino acid was estimated to be 38 fmol.
Bibliography:ObjectType-Article-1
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ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/28.3.527