CE-ICP-MS to probe Aβ1-42/copper (II) interactions, a complementary tool to study amyloid aggregation in Alzheimer's disease

Copper (II) ions appear to be involved in the Alzheimer's disease and seem to influence the aggregation of the amyloid-β1-42 (Aβ1-42) peptide. However, data are not conclusive and still not subject to consensus, copper (II) being suspected to either promote or inhibit aggregation. To address th...

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Bibliographic Details
Published inMetallomics Vol. 14; no. 1
Main Authors Duroux, C, Hagège, A
Format Journal Article
LanguageEnglish
Published England 13.01.2022
Subjects
amyloid-β
oligomerization
copper speciation
CE-ICP-MS hyphenation
labile complexes
voltage induced dissociation
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Summary:Copper (II) ions appear to be involved in the Alzheimer's disease and seem to influence the aggregation of the amyloid-β1-42 (Aβ1-42) peptide. However, data are not conclusive and still not subject to consensus, copper (II) being suspected to either promote or inhibit aggregation. To address this question, CE-ICP-MS (capillary electrophoresis-inductively coupled plasma-mass spectrometry) hyphenation was proposed as a complementary tool to follow the distribution of copper in the different oligomeric forms, at different substoichiometries and different incubation times. Results clearly indicated the formation of several negatively charged copper complexes and showed the enhancement of the aggregation rate with copper concentration. Moreover, the variations of copper (II) speciation suggest different aggregation pathway, even for substoichiometric ratios.
ISSN:1756-591X
DOI:10.1093/mtomcs/mfab075