Lipopolysaccharides from six strains of Acetobacter diazotrophicus

Lipopolysaccharides from six nitrogen-fixing strains of Acetobacter diazotrophicus (PR2, PAL3, PAL5, PR4, PR14, PR20), isolated from sugarcane, were purified by phenol-water extraction and ultracentrifugation. The relatively large molecular mass observed by SDS-PAGE indicated that the lipopolysaccha...

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Published inFEMS microbiology letters Vol. 132; no. 1/2; pp. 45 - 50
Main Authors Fontaine, T, Stephan, M.P, Debarbieux, L, Previato, J.O, Mendonca-Previato, L
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.10.1995
Blackwell
Wiley-Blackwell
Subjects
6‐Deoxy‐hexose
Acetobacter
Acetobacter diazotrophicus
Bacteriology
Biological and medical sciences
chemical composition
chemotaxonomy
Fundamental and applied biological sciences. Psychology
Life Sciences
Lipopolysaccharide
lipopolysaccharides
Microbiology
Morphology, structure, chemical composition
nitrogen-fixing bacteria
Ribose
Saccharum officinarum
strain differences
strains
Sugarcane
Acetobacteraceae
Purification
Structure activity relation
Ribose
Chemotaxonomy
Lipopolysaccharide
Bacteria
Chemical composition
Acetobacter
Strain
Acetobacter diazotrophicus
Sugarcane
6-Deoxy-hexose
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Summary:Lipopolysaccharides from six nitrogen-fixing strains of Acetobacter diazotrophicus (PR2, PAL3, PAL5, PR4, PR14, PR20), isolated from sugarcane, were purified by phenol-water extraction and ultracentrifugation. The relatively large molecular mass observed by SDS-PAGE indicated that the lipopolysaccharides of each strain possessed an O-side chain. Analysis of each lipopolysaccharide by colorimetric assays and by gas liquid chromatography/mass spectrometry combination showed that the core and lipid A composition was similar for all strains, containing 3-deoxy-D-manno-2-octulosonic acid, glucosamine and fatty acid (16-0, 3-OH-14, 2-OH-16:0, 3-OH-16:0). The neutral sugar composition showed the predominance of 6-deoxy-hexose (rhamnose and fucose) and ribose, in comparison with hexose (glucose, galactose, mannose). The presence of 6-deoxy-hexose and ribose containing O-side chains is discussed as a way of discriminating A. diazatrophicus from other Acetobacter species.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1995.tb07808.x