ALKBH10B, an mRNA m6A Demethylase, Modulates ABA Response During Seed Germination in Arabidopsis
As the most abundant and reversible chemical modification in eukaryotic mRNA, the epitranscriptomic mark N 6 -methyladenine (m 6 A) regulates plant development and stress response. We have previously characterized that ALKBH10B is an Arabidopsis mRNA m 6 A demethylase and regulates floral transition...
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Published in | Frontiers in plant science Vol. 12; p. 712713 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
27.07.2021
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Subjects | |
Online Access | Get full text |
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Summary: | As the most abundant and reversible chemical modification in eukaryotic mRNA, the epitranscriptomic mark
N
6
-methyladenine (m
6
A) regulates plant development and stress response. We have previously characterized that ALKBH10B is an Arabidopsis mRNA m
6
A demethylase and regulates floral transition. However, it is unclear whether ALKBH10B plays a role in abiotic stress response. Here, we found that the expression of
ALKBH10B
is increased in response to abscisic acid (ABA), osmotic, and salt stress. The
alkbh10b
mutants showed hypersensitive to ABA, osmotic, and salt stress during seed germination. Transcriptome analysis revealed that the expression of several ABA response genes is upregulated in
alkbh10b-1
than that of wild type, indicating ALKBH10B negatively affects the ABA signaling. Furthermore, m
6
A sequencing showed that ABA signaling genes, including
PYR1
,
PYL7
,
PYL9
,
ABI1
, and
SnRK2.2
are m
6
A hypermethylated in
alkbh10b-1
after ABA treatment. Taken together, our work demonstrated that ALKBH10B negatively modulates ABA response during seed germination in Arabidopsis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Byeong-ha Lee, Sogang University, South Korea These authors have contributed equally to this work Reviewed by: Hunseung Kang, Chonnam National University, South Korea; Wilco Ligterink, KeyGene, Netherlands This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.712713 |