Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes

• Published in: Cell biology and toxicology Vol. 24; no. 1; pp. 19 - 25
• Main Authors: Rahman, M. T., De Ley, M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2008; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biosynthesis; Blood platelets; Blood Platelets - cytology; Blood Platelets - drug effects; Blood Platelets - metabolism; Cell Biology; Cellular biology; Enzymes; Gene Expression Regulation - drug effects; Humans; Immunohistochemistry; Infant, Newborn; Integrin beta3 - metabolism; Life Sciences; Megakaryocytes - cytology; Megakaryocytes - drug effects; Megakaryocytes - metabolism; Metallothionein - genetics; Metallothionein - metabolism; Original Article; Pharmacology/Toxicology; Protein Isoforms - genetics; Protein Isoforms - metabolism; Protein Transport - drug effects; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stem Cells - drug effects; Stem Cells - metabolism; Zinc; Zinc - pharmacology; MT isogene; Cord blood; Zinc; MACS; Nuclear-MT
• ISSN: 0742-2091; 1573-6822
• DOI: 10.1007/s10565-007-9012-3

The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61 + megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61 + cells at 48 h post-treatment with 100 μmol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar cell populations left untreated with zinc.