A 30-kDa protein is disulfide linked to IgM on normal and neoplastic murine B lymphocytes

• Published in: European journal of immunology Vol. 16; no. 1; p. 113
• Main Authors: Haustein, D, Von der Ahe, D
• Format: Journal Article
• Language: English
• Published: Germany 01.01.1986
• Subjects: Animals; B-Lymphocytes - ultrastructure; Cell Membrane - ultrastructure; Disulfides; Immunoglobulin M - metabolism; Membrane Proteins - metabolism; Mice; Molecular Weight; Receptors, Antigen, B-Cell - metabolism
• ISSN: 0014-2980; 1521-4141
• DOI: 10.1002/eji.1830160122

Four cloned continuously cultured mouse B lymphoma cells of the lines WEHI-279, BCL1 and 38C-13 were used to study the surface presentation of IgM. Cells of all lines expose, in addition to mu 2L2, various, disulfide-linked subunits as already shown for normal B lymphocytes (Koch, N. and Haustein, D., Mol. Immunol. 1982. 19:477). In contrast to normal B cells, B lymphoma cells also carry IgM structures of higher molecular weight than mu 2L2 on their surface. Furthermore, B lymphoma cells of all investigated lines expose a mu 2L2 structure to which a 30-kDa protein is disulfide-linked. This 30-kDa protein could also be identified on normal mouse B lymphocytes when more drastic conditions were employed. mu 2L2 disulfide linked to this 30-kDa protein is the main IgM structure on normal and neoplastic B cells whereas only small amounts of mu 2L2 (unlinked to this protein) can be detected.