Phenolic Compounds from Japanese Anise (Illicium anisatum L.) Leaves

• Published in: Journal of the Korean Wood Science and Technology Vol. 47; no. 1; pp. 1 - 7
• Main Authors: Shinn, Seong-whan, Min, Hee-Jeong, Bae, Young-Soo
• Format: Journal Article
• Language: English; Korean
• Published: 한국목재공학회 2019
• Subjects: column chromatography; EtOAc soluble; H2O soluble; Japanese anise (llicium anisatum L); leaves extracts; 임학; Japanese anise (llicium anisatum L); leaves extracts; H_2O$ soluble; column chromatography; EtOAc soluble
• ISSN: 1017-0715; 2233-7180
• DOI: 10.5658/WOOD.2019.47.1.1

Japanese anise (Illicium anisatum L.) leaves were collected and ground after drying, then immersed with 50 % aqueous acetone for 3 days. After filtration, the extracts were fractionated with n-hexane, chloroform (CHCl3), ethylacetate (EtOAc) and H2O, and then freeze dried after concentration. A portion of EtOAc (3.12 g) and H2O (6.08 g) soluble fractions were chromatographed on a Sephadex LH-20 column with various aqueous MeOH solution to isolate the compounds. Compound 1 ((+)-catechin) was isolated from EtOAc soluble fraction. Compounds 2 (quercetin), 3 (quercitrin) and 4 (2''-O-rhamnosylvitexin) were isolated from H2O soluble fraction. For the first time, quercitrin (3) and 2''-O-rhamnosylvitexin (4) of the isolated compounds were obtained from the extracts of japanese anise leaves.