In vitro cell proliferation and proteoglycan synthesis of rabbit meniscal fibrochondrocytes as a function of age and sex

Meniscal fibrochondrocytes from male and female New Zealand white rabbits, ages 6, 12, and 24 months, were grown in primary and secondary monolayer cell culture. Neither age nor sex affected the majority of their cell culture characteristics. Cells from young males (6 months old) synthesized greater...

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Bibliographic Details
Published inArthritis and rheumatism Vol. 29; no. 8; p. 1010
Main Authors Webber, R J, Zitaglio, T, Hough, Jr, A J
Format Journal Article
LanguageEnglish
Published United States 01.08.1986
Subjects
Adult
Age Factors
Animals
Cell Division
Cells, Cultured
Chromatography, Gel
Female
Fibroblast Growth Factors - pharmacology
Humans
In Vitro Techniques
Male
Menisci, Tibial - cytology
Menisci, Tibial - drug effects
Menisci, Tibial - metabolism
Menisci, Tibial - physiology
Proteoglycans - biosynthesis
Proteoglycans - isolation & purification
Rabbits
Sex Factors
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Summary:Meniscal fibrochondrocytes from male and female New Zealand white rabbits, ages 6, 12, and 24 months, were grown in primary and secondary monolayer cell culture. Neither age nor sex affected the majority of their cell culture characteristics. Cells from young males (6 months old) synthesized greater amounts of sulfated proteoglycans than did those from young females, but by 2 years of age, this result was reversed. All age groups synthesized 2 classes of proteoglycans, based on hydrodynamic size, but the ratio of the 2 classes changed as a function of age. Overall, the meniscal fibrochondrocytes from both skeletally immature and skeletally mature rabbits of both sexes were capable of proliferation and matrix synthesis in vitro.
ISSN:0004-3591
DOI:10.1002/art.1780290810