Prebiotic-mediated gastroprotective potentials of three bacterial levans through NF-κB-modulation and upregulation of systemic IL-17A

• Published in: International journal of biological macromolecules Vol. 250; p. 126278
• Main Authors: Saleh, Shireen A.A., Shawky, Heba, Ezzat, Asmaa, Taie, Hanan A.A., Salama, Bassem, El-Bassyouni, Gehan T., El Awdan, Sally A., Awad, Ghada E.A., Hashem, Amal M., Esawy, Mona A., Abdel Wahab, Walaa A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2023
• Subjects: Honey isolates; Immunomodulation; Levan; Peptic ulcer; Prebiotics; Systemic inflammation; Peptic ulcer; Honey isolates; Levan; Immunomodulation; Systemic inflammation; Prebiotics
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.126278

This study aimed to investigate whether the gastroprotective effects of three types of bacterial levans are correlated with their prebiotic-associated anti-inflammatory/antioxidant potentials. Three levans designated as LevAE, LevP, and LevZ were prepared from bacterial honey isolates; purified, and characterized using TLC, NMR, and FTIR. The anti-inflammatory properties of levan preparations were assessed in LPS-stimulated RAW 264.7 cell lines, while their safety and gastroprotective potentials were assessed in Wistar rats. The three levans significantly reduced ulcer number (22.29–70.05 %) and severity (31.76–80.54 %) in the ethanol-induced gastric ulcer model compared to the control (P < 0.0001/each), with the highest effect observed in LevAE and levZ (200 mg/each) (P < 0.0001). LevZ produced the highest levels of glutathione; catalase activity, and the lowest MDA levels (P = 0.0001/each). The highest anti-inflammatory activity was observed in LevAE and levZ in terms of higher inhibitory effect on IL-1β and TNF-α production (P < 0.0001 each); COX2, PGE2, and NF-κB gene expression. The three levan preparations also proved safe with no signs of toxicity, with anti-lipidemic properties as well as promising prebiotic activity that directly correlated with their antiulcer effect. This novel study highlights the implication of prebiotic-mediated systemic immunomodulation exhibited by bacterial levans that directly correlated with their gastroprotective activity. •Three types of levans were prepared from honey bacterial isolates and characterized.•The three levans significantly reduced ulcer number/severity in gastric ulcer model.•Prepared levans inhibited the inflammatory cascade on the transcriptional level.•All levans showed promising anti-lipidemic properties with no signs of toxicity.•The gastroprotective effect of prepared levans was largely prebiotic-specific.