Fluorometric Determination of Cyanate in Plasma by Conversion to 2,4(1 H,3 H)-Quinazolinedione and Separation by High-Performance Liquid Chromatography

A fluorometric high-performance liquid-chromatographic method is described for the determination of cyanate in human plasma. The method is based on the determination of cyanate with 2-aminobenzoic acid (anthranilic acid), leading to a stable cyclic fluorescent product, 2,4(1 H,3 H)-quinazolinedione....

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Bibliographic Details
Published inAnalytical biochemistry Vol. 211; no. 1; pp. 23 - 27
Main Authors Lundquist, P., Backmangullers, B., Kagedal, B., Nilsson, L., Rosling, H.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.05.1993
Elsevier
Subjects
Adult
Analysis of complex biological substances
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Blood and biological fluids
Chromatography, High Pressure Liquid
Cyanates - blood
Cyanates - chemistry
Female
Fluorometry
Fundamental and applied biological sciences. Psychology
Humans
Male
Middle Aged
Quinazolines - analysis
Reference Values
Reproducibility of Results
Sensitivity and Specificity
Smoking - blood
Urea - chemistry
Human
Analysis method
Fluorescent labelling
Cyanates
HPLC chromatography
Derivatization
Fluorescence spectrometry
Quantitative analysis
Blood plasma
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Summary:A fluorometric high-performance liquid-chromatographic method is described for the determination of cyanate in human plasma. The method is based on the determination of cyanate with 2-aminobenzoic acid (anthranilic acid), leading to a stable cyclic fluorescent product, 2,4(1 H,3 H)-quinazolinedione. The fluorescent product was extracted with ethyl acetate, passed through a cation-exchange resin to eliminate the excess of determination reagent, and then concentrated on a Sep-Pak C-18 cartridge before reversed-phase chromatography and fluorometric detection. The precision of the method was satisfactory (coefficient of variation 2.3%) and the analytical recovery was quantitative (103%). Detection limit was found to be 8 nmol/liter from a 1-ml plasma sample. Cyanate in plasma was stable for 3 months when stored in liquid nitrogen. The mean and SD cyanate level in human plasma from 17 healthy nonsmoking subjects was 45 ± 21 nmol/liter.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1993.1226