Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii

Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of prot...

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Bibliographic Details
Published inBiotechnology techniques Vol. 13; no. 6; pp. 385 - 390
Main Authors Cavalitto, S.F, Hours, R.A, Mignone, C.F
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 01.06.1999
Subjects
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
enzyme activity
Fundamental and applied biological sciences. Psychology
Geotrichum klebahnii
Miscellaneous
Mission oriented research
polygalacturonase
Fungi
Analysis method
Enzymatic activity
Enzyme
Glycosidases
Hydrolases
Pectin
Fungi Imperfecti
Polygalacturonase
O-Glycosidases
Thallophyta
Quantitative analysis
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Summary:Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g l super(-1), reaction time of 10 min and up to 0.17 PGase units per test tube.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008917912296